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June 2, 2005
Antitumor Effect of a Combination of Lysine, Proline, Arginine, Ascorbic Acid, and Green Tea Extract on Pancreatic Cancer Cell Line MIA PaCa-2.
Topics: Pancreatic CancerResearchers report that the formulation of green tea extract, lysine, proline, and ascorbic acid, have tested as a promising adjunct to standard treatment of pancreatic cancer.
Current treatment of pancreatic cancer is generally associated with a poor prognosis, even if diagnosed early, owing to its aggressive rate of metastasis and non-responsiveness to chemotherapy and radiotherapy once metastasis occurs.
The matrix metalloproteinase(MMP) family of enzymes contribute to both normal and pathological tissue remodeling. MMPs play a key role in the migration of normal and malignant cells through the body and also act as regulatory molecules, both by functioning in enzyme cascades and by processing matrix proteins, cytokines, growth factors and adhesion molecules to generate fragments with enhanced or reduced biological effects. MMPs have received much attention in recent years for their role in various malignancies, and have been implicated in tumor invasion, metastasis, and angiogenesis. Investigators, prompted by the reported antitumor properties of ascorbic acid, lysine, proline, and green tea extract, researched the effect of a combination of lysine, proline, arginine, ascorbic acid, and green tea extract on pancreatic cancer cell line MIA PaCa-2 for viability(here, here), MMP expression, invasion, and morphology.
Viability was evaluated based on cell proliferation by MTT assay and MMP expression in condition media by gelatinase zymography. Invasion through Matrigel was assayed and morphology was observed by hematoxylin and eosin staining. Data was analyzed by an independent sample "t" test.
The nutrient mixture did not inhibit cell proliferation at 10 micro-g/mL and exhibited a dose-dependent antiproliferative effect with maximum inhibition of 38% over the control at 1000 micro-g/mL. Zymography demonstrated production of only MMP-9, which showed a dose-dependent decreased expression that was abolished at 100 micro-g/mL of nutrient mixture. Invasion through Matrigel was inhibited at 10, 50, 100, and 500 micro-g/mL by 66%, 66%, 87% and 100%, respectively. hematoxylin and eosin staining did not indicate changes even at the highest concentration of the nutrient mixture.
The results suggest that the formulation of green tea extract, lysine, proline, and ascorbic acid, tested as a promising adjunct to standard treatment of pancreatic cancer, by inhibiting MMP expression and invasion without toxic effects - all very important parameters in cancer metastasis.
Optional reading(looks like good method but the article isn't peer reviewed):
Cross posted by Hyscience
Posted by Richard at June 2, 2005 12:27 PM
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